1.   (Fixative Solution)
20g 5-Sulfosalicylic Acid
dissolved in 1L distilled water


2. (Citric Acid/Phosphate Buffer)

Step A.
71.64 g Na2HPO4*12H2O
        -or-
35.61 g Na2HPO4*2H2O

Dissolved in distilled water. 
Make up to 1L in volumetric flask.
Shake vigorously to dissolve.

Step B.
21.01 g Citric acid monohydrate

Dissolved in distilled water.
Make up to 1L in volumetric flask.
Shake vigorously to dissolve.

Step C.

223 ml Na2HPO4*12H2O
  -or- Na2HPO4*2H2O
       from Step A.
177 ml Citric acid solution
       from Step B.


3.  (Working Solution)

1.25 g ABTS dissolved in 
250 ml Citric Acid/Phosphate Buffer

IMMERSION METHOD

1. 
Soak item in Fixative Solution for
3 min., then distilled water rinse.

2. (Activated Working Solution)

Using the following ratio:
 50 ml ABTS working solution
0.5 ml 27% Hydrogen peroxide

Combine and shake vigorously
in a stoppered bottle.

3.
Tray immersion of item into 
Activated Working Solution
for 5 min. Remove and rinse 
in distilled water.

4.
Allow item to air dry
under darkened lighting.
    
     -- or --
 
BLOTTING METHOD

1.
Place a dry, clean filter paper
over the region to be processed.

2.
Saturate the paper with the 
Fixative Solution using a pipette
for 3 min. Remove paper and rinse
the processed region with 
distilled water.

3.
Place another dry, clean filter paper
over the region to be processed.

4.
Vigorously shake the Activated
Working Solution. Saturate the paper
with the Activated Working Solution
using a pipette for 5 min. Remove 
paper and rinse the processed region
with distilled water.

5.
Allow item to air dry
under darkened lighting.