AMIDO BLACK - Methanol base

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Chemical Name:
AMIDO BLACK - Methanol base
Surface Used On:
Blood-stained Porous & Non-porous surfaces.
Sensitive To:
Blood proteins.
Development Color: Method to Record: Hazard: Protective Clothing: Fume Hood Use:

Deep blue.

Yes
Reagent Characteristics

Development Complete When:

Source of Error:

Incompatibilities:

Precautions:

Storage Container:

Safety:

Recommendations:


Similar Reagents



Sequential Reagents
(Not necessarily in this order.)



Abridged Reagent Sequence


Visual Examination
|
Forensic Light
|
Ultra-violet light
|
** Amido Black-Methanol base **
|
Forensic Light

AMIDO BLACK - Methanol base
  &bnsp; Formula
DEVELOPER  SOLUTION:

  2 g  Amido black dye
100 ml Glacial acetic acid
900 ml Methanol

(Combine and mix with a
stirring device for 
30 minutes.)

RINSE SOLUTION:

100 ml Glacial acetic acid
900 ml Methanol

FINAL RINSE:

1 liter distilled water
PROCEDURE OF APPLICATION

1. Be certain that the blood is
'dried' prior to application.

2a.
Squeegee bottle application -

Apply for 30 to 90 seconds. 
A squeegee bottle is used 
to apply rinse.
     
	-- or --

2b.
Tray immersion -

Immerse into dye solution 
30 to 90 seconds.
Immerse into a tray of
rinse solution for 1 minute.

3.
Apply the final water rinse.

4. 
Allow the item to air dry.


Ridge Detail Visualized by:

Visible chemical/stain reaction.


Reagent Applicabilities:

Blood Enhancement
Non-Porous surfaces


Other Chemical Name(s):

Naphthol Blue Black
Naphthalene 12B
Acid Black 1, 10A, 10B
Acid Black 12B, 10BA
Eriosin Blue Black B
Acidal Black 10B


Working Solution Shelf-life:

Indefinite

Process Summary:
A dye staining process, followed by rinse procedures, that is used to enhance detail in faint bloody impressions. Bloody impressions should be 'dried' prior to staining with this reagent.
Accepted Deviations:
Development time may be shortened if the blood impression or the underlying surface strongly absorbs the dye.
Supporting Reference Materials:
1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
2. "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.
3. Lee, H.C. & Gaensslen, R.E. (1991),"Advances in Fingerprint Technology", Elsevier, pg.86.
4. Slater, J., "Techniques for the Enhancement of 2-Dimensional Footwear Impressions in Blood", Forensic Services Div., May, 1995.
5. Navarro, R.L., "Chemical Enhancement of Questioned Footwear Impressions", North Carolina State Bureau of Inv., 1992.

Return to: Main Page   ||  Non-Porous Surfaces  ||  Blood Enhancement Techniques  ||  Metal Surfaces  ||  Glass Surfaces  ||  Plastic Surfaces  ]