SAFRANIN O

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Chemical Name:
SAFRANIN O
Surface Used On:
Non-porous surfaces.
Sensitive To:
Cyanoacrylate deposit.
Development Color: Method to Record: Hazard: Protective Clothing: Fume Hood Use:

Yellow fluorescence

Orange filter

Yes
Reagent Characteristics

Development Complete When:

Source of Error:

Incompatibilities:

Precautions:

Storage Container:

Safety:

Recommendations:


Similar Reagents



Sequential Reagents
(Not necessarily in this order.)



Abridged Reagent Sequence


Visual Examination
|
Forensic Light
|
Cyanoacrylate
|
** Safranin O **
|
Forensic Light
|
R.A.M.
|
Forensic Light

SAFRANIN O FORMULA:

   1  g Safranin O powder 

1000 ml Methanol 

Combine the above and stir using a
magnetic stirring device.

PROCEDURE OF APPLICATION

1.
Spray, immerse, or use a 
squirt bottle to apply the 
Safranin O solution to the item.

2. 
Allow the item to air dry.

3.
Examination under a laser or 
Forensic Light Source around 
the 500 nm region. 
Use orange colored goggles.

4.
Photograph results using 
an orange or bandpass 
550(BP35) barrier filter.


Ridge Detail Visualized by:

Forensic Light Source induced.


Reagent Applicabilities:

Post-Cyanoacrylate
Non-Porous surfaces
Fluorescent technique


Other Chemical Name(s):

Basic Red 2
Brilliant safranin BR
Brilliant Safranin G
Calcozine red Y
Safrin T
Leather red HT


Working Solution Shelf-life:

Indefinite

Process Summary:
A fluorescent dye-stain used to enhance cyanoacrylate-developed latent prints. A fluorescent light source that will output light around 500 nm is required for this process.
Accepted Deviations:
A rinse of methanol may be used to remove excess dye stain from the item. A heat gun may be used to dry the item before viewing with a Forensic Light Source.
Supporting Reference Materials:
1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
2. "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.

Return to: Main Page   ||  Non-Porous Surfaces  ||  Fluorescent Techniques  ||  Post-cyanoacrylate  ||  Metal Surfaces  ||  Glass Surfaces  ||  Plastic Surfaces  ||  Cartridges  ]