Safranin O

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Chemical Name:
SAFRANIN O

Surface Used On:
Non-porous surfaces.

Sensitive To:
Cyanoacrylate deposit.

Development Color:	Method to Record:	Hazard:	Protective Clothing:	Fume Hood Use:
Yellow fluorescence	Orange filter			Yes

Reagent Characteristics

Development Complete When:

Source of Error:

Incompatibilities:

Precautions:

Storage Container:

Safety:

Recommendations:

Similar Reagents

SAFRANIN O FORMULA:


   1  g Safranin O powder 

1000 ml Methanol 

Combine the above and stir using a
magnetic stirring device.

PROCEDURE OF APPLICATION


1.
Spray, immerse, or use a 
squirt bottle to apply the 
Safranin O solution to the item.

2. 
Allow the item to air dry.

3.
Examination under a laser or 
Forensic Light Source around 
the 500 nm region. 
Use orange colored goggles.

4.
Photograph results using 
an orange or bandpass 
550(BP35) barrier filter.

Ridge Detail Visualized by:

Forensic Light Source induced.

Reagent Applicabilities:

Post-Cyanoacrylate
Non-Porous surfaces
Fluorescent technique

Other Chemical Name(s):

Basic Red 2
Brilliant safranin BR
Brilliant Safranin G
Calcozine red Y
Safrin T
Leather red HT

Working Solution Shelf-life:

Indefinite

Process Summary:
A fluorescent dye-stain used to enhance cyanoacrylate-developed latent prints. A fluorescent light source that will output light around 500 nm is required for this process.

Accepted Deviations:
A rinse of methanol may be used to remove excess dye stain from the item. A heat gun may be used to dry the item before viewing with a Forensic Light Source.

Supporting Reference Materials:
1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
2. "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.

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