CHESAPEAKE BAY DIVISION - IAI
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Nickel Nitrate

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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing: 
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 Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagents
Formula
3 g Nickle nitrate dissolved in 300 ml Ethyl ether. Use magnetic stirrer until completely dissolved.​



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Procedure of Application
Ninhydrin Treated Items:
  1. Spray the item lightly.
  2. Air-dry the item.
  3. Process & dry a second time.
  4. Oven bake at 80 - 100 degrees C at 65% humidity for 20 minutes. 
  5. View under a forensic light source around 530 nm. A fluorescent color is not produced, but the absorbed light may yield better detail.



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Development Complete When
The Ruhemann's purple color is shifted to a orange-red color.

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Source of Error
Areas deeply stained Ruhemann's purple may yield little detail.
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Incompatibilities
Items that have not been treated with Ninhydrin.

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Precautions
Spray Nickle nitrate LIGHTLY onto the item.  Avoid visibly wetting the surface with the reagent solution.

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Storage Container
Dark stoppered glass bottles.

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Safety
Use a fume hood when applying the reagent. 
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Recommendations
Use a fine-mist aerosol sprayer to apply the reagent solution.

Use a forensic light source to induce fluorescence of the item or to have the nickle nitrate to darken by absorbing the light.




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Similar Reagent
  • Zinc Chloride

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Sequential Reagents
  • Physical Developer
  • Sodium Hydrochlorite
  • Silver Nitrate

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Chemical Name
Nickel Nitrate
​Surface Used On
Porous Surfaces
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Sensitive To
Ninhydrin Compounds
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Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light
  3. Ninhydrin
  4. Nickel Nitrate
  5. Forensic Light​​
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Ridge Detail Visualized by:
Visible chemical/stain reaction
Forensic Light Source induced​

​​Reagent Applicabilities:
Porous sufaces
Post-Ninhydrin

Other Chemical Name(s):

None
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Process Summary:
Nickel nitrate is applied as post-ninhydrin treatment in order to improve the contrast of the ridge detail for viewing and photography. Two methods may be applied, the background may be made to fluoresce, or, the treated ridge detail may be made to absorb green-colored light around 530 nm. 

Accepted Deviations:
​None

Supporting Reference Materials:
  1. Advances in Fingeprint Technology 2nd. Ed., Lee, H.C. & Gaensslen, R.E., CRC Press, Boca Raton, FL., 2001.

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
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