CHESAPEAKE BAY DIVISION - IAI
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Amido Black - Methanol Base

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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing: 
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 Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Formula
Developer Solution:
   1.   2 g Amido black dye
   2.  100 ml Glacial acetic acid
   3.  900 ml Methanol (Combine and mix with a stirring     
        device for 30 minutes.)

Rinse Solution:
   1.  100 ml Glacial acetic acid
   2. 900 ml Methanol

​Final Rinse:
   1.  1 liter distilled water

Procedure of Application
   1. Be certain that the blood is 'dried' prior to application.   
   2a. Squeegee bottle application -

Apply for 30 to 90 seconds. A squeegee bottle is used to apply rinse.

-- or --
  
   2b. Tray immersion - Immerse into dye solution 30 to 90
          seconds. Immerse into a tray of rinse solution for 1
          minute.
   3. Apply the final water rinse.
   4. Allow the item to air dry.
Development Complete When
Maximum contrast is achieved of the blue development color upon repeated staining and rinsing procedures.

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Source of Error
Cyanoacrylate fuming may be detrimental to this reagent.

Excessively blood-stained items will obliterate detail.
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Incompatibilities
Painted surfaces may be deteriorated by the methanol in the working and rinse solutions.

Excessively blood-stained items and porous surfaces that strongly absorb the dye will yield little contrast to the developed detail.



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Precautions
This process is detrimental to some biological examinations.

Don't let the evidence over-develop in the working solution.
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Storage Container
Dark or Clear stoppered glass or plastic bottles.


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Safety
Fume hood use is required to prepare and apply the working solution.
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Recommendations
Be certain that the blood is "dried" to the surface prior to applying this reagent.






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Similar Reagent
  • Amido Black - Water Base
  • Crowle's Double Stain
  • D.A.B.
  • Leucocrystal Violet

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Chemical Name
Amido Black - Methanol Base
​Surface Used On
Blood-stained Porous & Non-Porous Surfaces
​
Sensitive To
Proteins in Blood
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Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light
  3. Ultra-Violet Light
  4. Amido Black-Methanol Base
  5. Forensic Light
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Ridge Detail Visualized by:
Visible chemical/stain reaction​

​​Reagent Applicabilities:

Non-Porous Surfaces
Blood Enhancement

Other Chemical Name(s):

Naphthol Blue Black
Naphthalene 12B
Acid Black 1, 10A, 10B
Eriosin Blue Black B
Acidal Black 10B


Working Solution Shelf-life:

Indefinite

Process Summary:
A dye staining process, followed by rinse procedures, that is used to enhance detail in faint bloody impressions. Bloody impressions should be "dried" prior to staining with this reagent. 

Accepted Deviations:
Development time may be shortened if the blood impression or the underlying surface strongly absorbs the dye. ​

Supporting Reference Materials:
  1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
  2. "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.
  3. Lee, H.C. & Gaensslen, R.E. (1991),"Advances in Fingerprint Technology", Elsevier, pg.86.
  4. Slater, J., "Techniques for the Enhancement of 2-Dimensional Footwear Impressions in Blood", Forensic Services Div., May, 1995.
  5. Navarro, R.L., "Chemical Enhancement of Questioned Footwear Impressions", North Carolina State Bureau of Inv., 1992.

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
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