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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing:
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Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagents
Formula
Procedure of Application
1. Solution A fixation 2-3 minutes, then water rinse. 2a. Tray immersion of item into working solution for 3 to 4 minutes. -- or -- 2b. Paper towel saturation of working solution for 3 minutes. 3. Distilled water rinse. Development Complete When
Enhancement of blood detail is noted. Source of Error
D.A.B. is a protein stain that is not specific for blood. Dye will also absorb to palmar sweat. Cyanoacrylate fuming has an adverse effect on D.A.B. Distilled water must be used. Incompatibilities
Porous items that strongly absorb the dye. Excessively blood-stained items. Cyanoacrylate fuming is detrimental to this process. Precautions
D.A.B. processing must be completed before processing with Amido Black. Use Distilled water only. Storage Container
Dark stoppered plastic bottles. Safety
D.A.B. spots are difficult to remove from skin or clothing Recommendations
Enhanced results that are exposed to direct sunlight should be photographed immediately, since photo ionization may produce unwanted background development. Similar Reagent
Sequential Reagents
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Chemical Name
Diaminobenzidine Surface Used On Blood-Stained Porous & Non-Porous Surfaces Sensitive To Proteins in Blood Abridged Reagent Sequence
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Ridge Detail Visualized by:
Visible chemical/stain reaction Reagent Applicabilities: Porous Surfaces Non-Porous Surfaces Blood Enhancement Other Chemical Name(s): 3,3'-Diaminobenzidine Tetrahydrochloride Working Solution Shelf-life: 48 hours if refrigerated |
Process Summary:
A peroxidase reagent which is colorless, but becomes strongly visible when reacted with blood. May be used in conjunction with Amido Black. Good results achieved on some paper items. Ninhydrin has no effect on the D.A.B. process. Other blood enhancement colored protein stains may be used after D.A.B. Cyanoacrylate fuming is detrimental to D.A.B. processing.
A peroxidase reagent which is colorless, but becomes strongly visible when reacted with blood. May be used in conjunction with Amido Black. Good results achieved on some paper items. Ninhydrin has no effect on the D.A.B. process. Other blood enhancement colored protein stains may be used after D.A.B. Cyanoacrylate fuming is detrimental to D.A.B. processing.
Accepted Deviations:
D.A.B. can be applied in two general manners - by immersing the item in a D.A.B. solution, or, by a "tissue" method.
D.A.B. can be applied in two general manners - by immersing the item in a D.A.B. solution, or, by a "tissue" method.
Supporting Reference Materials:
- Sahs, P., "DAB: An Advancement in Blood Print Detection", J. Forensic. Ident., Vol. 42., No. 5,(1992), pg 412.
- Slater, J., "Techniques for the Enhancement of 2-Dimensional Footwear Impressions in Blood", Forensic Services Div., May, 1995.
- "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, FBI Laboratory, pg. 23, 1994.
- Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.