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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing:
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Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagents
Formula
Formula "A": 1. (Solution A) 10g 5-Sulfosalicylic Acid dissolved in 100 ml distilled water 2. (Solution B) add Solution A to 400 ml 3% Hydrogen Peroxide 3. (Working Solution) add .75 g Leucocrystal violet dye to Solution B stirring the mixture vigorously. Formula "B": 1. 10g 5-Sulfosalicylic Acid dissolved in 500 ml 3% Hydrogen Peroxide 2. add 3.7 g Sodium acetate 3. add 1.0 g Leucocrystal violet dye stirring the mixture vigorously. Procedure of Application
Spray the blood impression using a fine-mist sprayer. Development should occur in 30 seconds. Development Complete When
Rapid impression enhancement to a violet color from the colorless form of the reagent. Source of Error
Background development of the item may occur under intense light due to photoionization of the dye. L.C.V. may react to other substances not specific to blood. Incompatibilities
Porous items that strongly absorb the dye. Excessively blood-stained items. Cyanoacrylate fuming may be detrimental to this procedure. The D.A.B. process for blood enhancement should not be performed before this reagent is applied. Precautions
The application of excess reagent may result in leaching of the impression, therefore apply the reagent using the finest misting device available. Photograph any enhanced impressions subject to sunlight shortly after development, due to photoionization concerns. Storage Container
Dark stoppered glass or plastic bottles. Safety
The reagent should be prepared in a fume hood. Use in a well ventillated area. Recommendations
This is the reagent of choice when you want to process a large area without any concerns for cleaning up from any rinse solution. Similar Reagent
Sequential Reagents
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Chemical Name
Leucocrystal Violet Surface Used On Blood-Stained Surfaces Sensitive To Hemoglobin in Blood Abridged Reagent Sequence
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Ridge Detail Visualized by:
Visible chemical/stain reaction Reagent Applicabilities: Porous Surfaces Non-Porous Surfaces Blood Enhancement Post Ninhydrin Other Chemical Name(s): L.C.V. Working Solution Shelf-life: One (1) month |
Process Summary:
A quick and uncomplicated method to enhance blood through the catalytic oxidation of the dye, while simultaneously fixing and enhancing the blood impression.
Other blood enhancement techniques such as Amido Black may be applied after this technique.
A quick and uncomplicated method to enhance blood through the catalytic oxidation of the dye, while simultaneously fixing and enhancing the blood impression.
Other blood enhancement techniques such as Amido Black may be applied after this technique.
Accepted Deviations:
The working solution may be lightly blotted 30 seconds after its application. Once the impression is blotted, the reagent may be lightly applied again.
The working solution may be lightly blotted 30 seconds after its application. Once the impression is blotted, the reagent may be lightly applied again.
Supporting Reference Materials:
- Fisher, John F., "An Aqueous Leucocrystal Violet Enhancing Reagent for Blood Impressions".
- "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.
- Bodziak, William J., "Use of Leucocrystal Violet to Enhance Shoe Prints in Blood", Forensic Science International, Vol.82, No.1, Sept. 1996.