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Amido Black - Water Base

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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing: 
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 Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagents
Formula
1st Solution : (Citric Acid Stock)
38 g of Citric Acid 
2000 ml of Distilled water     

2nd Solution : (Developer Solution)
1000 ml of Citric Acid Stock 
2 g of Amido black powder
2 ml of Kodak 'PhotoFlo 600' solution

3rd Solution : (1st. Rinse)
1000 ml of Citric Acid Stock  

4th Solution : (2nd. Rinse)
Distilled water
Procedure of Application
1. Fix Blood Impression:
Immerse in Methanol - 1 hour - or - Immerse in 5-Sulfosalicylic acid.

2. Working Solution Application:
Squeeze bottle application of dye for 2 minutes. - or - Tray immersion of item into dye for 2 minutes.

3. Rinse Solutions:
Squeegee bottle used to apply rinse solution for 1 minute. - or - Tray immersion containing rinse, rock tray gently for 1 minute.
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Development Complete When
Upon completion of the rinse procedure(s), the enhancement of blood detail is satisfactory.

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Source of Error
The blood impression must be fixed (dried) before applying the working solution, or else the impression may 'dissipate'. 

Amido black is a protein stain that is not blood-specific.
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Incompatibilities
Blue-colored items and-or porous items that strongly absorb the dye.

Excessively blood-stained items may depict little contrast between the background and the developed detail.

Cyanoacrylate fuming is detrimental to this process.


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Precautions
Porous-surfaced items may become excessively stained.

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Storage Container
Dark or clear stoppered glass or plastic bottles.

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Safety
Use in a well-ventillated area.
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Recommendations
The blood impression must be completely dry before processing with this reagent.

Use metal containers for immersion or dipping.





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Similar Reagent
  • Amido Black - Methanol Base
  • Coomassie Blue
  • Crowle's Double Stain
  • D.A.B.
  • Leucocrystal Violet

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Sequential Reagents
  • Physical Developer













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Chemical Name
Amido Black - Water Base
​Surface Used On
Blood-stained Non-Porous Surfaces, especially plastics
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Sensitive To
Proteins in Blood
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Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light
  3. Ultra-Violet Light
  4. Amido Black - Water Base
  5. Forensic Light
  6. Physical Developer
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Ridge Detail Visualized by:
Visible chemical/stain reaction​

​Reagent Applicabilities:

Non-Porous Surfaces
Blood Enhancement
Post-Ninhydrin

Other Chemical Name(s):

Naphthol Blue Black
Naphthalene 12B
Acid Black 1, 10A, 10B
Acid Black 12B, 10BA
Eriosin Blue Black B
Acidal Black 10B


​Working Solution Shelf-life:

Twelve (12) Months - Unless Contaminated

Process Summary:
A dye staining process used to enhance the detail in faint bloody impressions.
The blood impressions are to be first "fixed" prior to the application of the dye.

Accepted Deviations:
Development time is reduced for background which absorb the dye. Tap water may be substituted for distilled water in the final rinse.

Supporting Reference Materials:
  1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
  2. "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.
  3. Advances in Fingeprint Technology 2nd. Ed., Lee, H.C. & Gaensslen, R.E., CRC Press, Boca Raton, FL., 2001.
  4. Manual of Fingerprint Development Techniques 2nd. Ed., Home Office - Police Scientific Development Branch, White Crescent Press, Ltd., Luton, England, 2001.
  5. Technical Notes, Lightning Powder Co. Inc., Salem, OR., 2001.

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
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