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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing:
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Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagents
Formula
1st Solution : (Citric Acid Stock) 38 g of Citric Acid 2000 ml of Distilled water 2nd Solution : (Developer Solution) 1000 ml of Citric Acid Stock 2 g of Amido black powder 2 ml of Kodak 'PhotoFlo 600' solution 3rd Solution : (1st. Rinse) 1000 ml of Citric Acid Stock 4th Solution : (2nd. Rinse) Distilled water Procedure of Application
1. Fix Blood Impression: Immerse in Methanol - 1 hour - or - Immerse in 5-Sulfosalicylic acid. 2. Working Solution Application: Squeeze bottle application of dye for 2 minutes. - or - Tray immersion of item into dye for 2 minutes. 3. Rinse Solutions: Squeegee bottle used to apply rinse solution for 1 minute. - or - Tray immersion containing rinse, rock tray gently for 1 minute. Development Complete When
Upon completion of the rinse procedure(s), the enhancement of blood detail is satisfactory. Source of Error
The blood impression must be fixed (dried) before applying the working solution, or else the impression may 'dissipate'. Amido black is a protein stain that is not blood-specific. Incompatibilities
Blue-colored items and-or porous items that strongly absorb the dye. Excessively blood-stained items may depict little contrast between the background and the developed detail. Cyanoacrylate fuming is detrimental to this process. Precautions
Porous-surfaced items may become excessively stained. Storage Container
Dark or clear stoppered glass or plastic bottles. Safety
Use in a well-ventillated area. Recommendations
The blood impression must be completely dry before processing with this reagent. Use metal containers for immersion or dipping. Similar Reagent
Sequential Reagents
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Chemical Name
Amido Black - Water Base Surface Used On Blood-stained Non-Porous Surfaces, especially plastics Sensitive To Proteins in Blood Abridged Reagent Sequence
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Ridge Detail Visualized by:
Visible chemical/stain reaction Reagent Applicabilities: Non-Porous Surfaces Blood Enhancement Post-Ninhydrin Other Chemical Name(s): Naphthol Blue Black Naphthalene 12B Acid Black 1, 10A, 10B Acid Black 12B, 10BA Eriosin Blue Black B Acidal Black 10B Working Solution Shelf-life: Twelve (12) Months - Unless Contaminated |
Process Summary:
A dye staining process used to enhance the detail in faint bloody impressions.
The blood impressions are to be first "fixed" prior to the application of the dye.
A dye staining process used to enhance the detail in faint bloody impressions.
The blood impressions are to be first "fixed" prior to the application of the dye.
Accepted Deviations:
Development time is reduced for background which absorb the dye. Tap water may be substituted for distilled water in the final rinse.
Development time is reduced for background which absorb the dye. Tap water may be substituted for distilled water in the final rinse.
Supporting Reference Materials:
- Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
- "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.
- Advances in Fingeprint Technology 2nd. Ed., Lee, H.C. & Gaensslen, R.E., CRC Press, Boca Raton, FL., 2001.
- Manual of Fingerprint Development Techniques 2nd. Ed., Home Office - Police Scientific Development Branch, White Crescent Press, Ltd., Luton, England, 2001.
- Technical Notes, Lightning Powder Co. Inc., Salem, OR., 2001.