CHESAPEAKE BAY DIVISION - IAI
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Acid Yellow 7

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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing: 
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 Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagent
Formula
Acid Yellow 7 Pre-made Solution
  1. 4% Acid Yellow 7
  2. 84% Water
  3. 10% Acetic Acid
  4. 2% Formic Acid​







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Procedure of Application
  1. Fixing the Blood Print - Fixing is not required, but is recommended. A solution of 5% 5-sulfo-salicylic acid would be misted (pump sprayer) over the enhancement area. After fixing, allow 15 minutes for the fixative to dry. When the blood is relatively fresh, you will notice that fixing changes its color from dark red to dark brown.
  2. Staining the Blood Print - Once the print is fixed, it can be stained with Acid Yellow 7 Solution. Use the pump spray to mist the solution over the blood print. DO NOT SPRAY DIRECTLY AT PRINT, hover over the space being treated, spraying parallel to the surface. After spraying, allow the stain to set, usually 5-10 minutes.
  3. Rinse the Print - After the stain has set, rinse the area to remove background. This can be done with water or white vinegar. If the print is on a horizontal surface, gently pour water across the stained print and use absorbent paper to carefully blot the residual dye away from the background.​
Development Complete When
Prints will be yellow in color, but will fluoresce a bright yellow under alternate light sources in the blue to blue/green range of 455nm to 470nm. View with an orange barrier filter. Photograph with a comparable orange filter on the lens. 









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Source of Error
Prints wash away due to fixative not being applied.

Cyanoacrylate fuming may be detrimental to this reagent.
Excessively blood-stained items will obliterate detail.










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Incompatibilities
Excessively blood-stained items and porous surfaces that strongly absorb the dye will yield little contrast to the developed detail.

Porous items will get destroyed by acid content in the solution and    waxed items may get eaten away or destroyed as well.








Precautions
use in fume hood, wear gloves and goggles due to its potential to be a lung and eye irritant.












Storage Container
Store in Flammable cabinet in the bottle it comes in.













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Safety
Fume hood is required to apply solution. Safety coat and goggles to be worn.




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Recommendations
Fix blood prior to use.



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Similar Reagent
  • Amido Black - Water Base
  • Amido Black - Methanol Base
  • Crowle's Double Stain
  • D.A.B
  • Leucocrystal Violet





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Sequential Reagent
None.









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Chemical Name
Acid Yellow 7
​Surface Used On
Blood-stained Non-porous Surfaces
​
Sensitive To
Blood Proteins
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Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light/UV light
  3. 5% Sulfosalicylic Acid (Blood Fixative)
  4. Acid Yellow 7​
  5. Forensic Light
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Ridge Detail Visualized by:
Blue/Green Light with Orange Filter (455nm-470nm)

Reagent Applicabilities:
Non-Porous Surfaces
Blood Enhancement

Other Chemical Name(s):
N/A

​Working Solution Shelf-life:
1 year from opening, unless otherwise indicated by manufacturer

Process Summary:
A dye staining process preceded by a blood fixative (5% Sulfosalicylic Acid), that is used to enhance detail in faint bloody impressions. 

Accepted Deviations:
Blood fixative not required if the premade Acid Yellow 7 contains 5% Sulfosalicylic Acid.​​

Supporting Reference Materials:
  1. Enotes.com Inc. World of Forensic Science/Alternate Light Source Analysis, <http://www.enotes.com/forensic-science/alternate-light-source-analysis>. February 7, 2011
  2. Sears, V.G.; Butcher, C.P.G.; Fitzgerald, L.A. "Enhancement of Fingerprints in Blood, Part 3: Reactive Techniques, Acid Yellow 7, and Process Sequences." Journal of Forensic Identification 2005, Vol. 55, No. 6, p. 741-763

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
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