CHESAPEAKE BAY DIVISION - IAI
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Safranin O

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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing: 
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 Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagent
Formula
  1. 1 g Safranin O powder
  2. 1000 ml Methanol
  3. Combine the above and stir using a magnetic stirring device.









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Procedure of Application
  1. Spray, immerse, or use a squirt bottle to apply the Safranin O solution to the item.
  2. Allow the item to air dry.
  3. Examination under a laser or Forensic Light Source around the 500 nm region. Use orange colored goggles.
  4. Photograph results using an orange or bandpass 550(BP35) barrier filter.




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Development Complete When
The cyanoacrylate has absorbed the dye stain.







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Source of Error
Surfaces that absorb too much dye stain will fluoresce too brilliantly to be effective for photography.






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Incompatibilities
Items that inherently fluoresce around the 500 nm range will interfere with the dye stain fluorescence .









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Precautions
Avoid excess build-up of cyanoacrylate, since this may result in ridge detail depicting little contrast to the strongly fluorescent surface.


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Storage Container
Dark or Clear stoppered glass or plastic bottles.







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Safety
Fume hood use is required when preparing and applying the reagent.






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Recommendations
This dye-stain is effective at the low 500 nm region of forensic light source illumination.





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Sequential Reagent
​Not Necessarily in this Order:​
  • M.R.M. 10
  • R.A.M.
  • R.A.Y.






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Chemical Name
Safranin O
​Surface Used On
Non-Porous Surfaces
​
Sensitive To
Cyanoacrylate Deposit
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Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light
  3. Cyanoacrylate Fuming
  4. Safranin O
  5. Forensic Light​
  6. R.A.M.
  7. Forensic LIght
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Ridge Detail Visualized by:
Forensic Light Source induced
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Reagent Applicabilities:
Non-Porous Surfaces
Fluorescent Technique
Post Cyanoacrylate

Other Chemical Name(s):

Basic Red 2
Brilliant safranin BR
Brilliant Safranin G
Calcozine red Y
Safrin T
Leather red HT
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Working Solution Shelf-life:
Indefinite

Process Summary:
A fluorescent dye-stain used to enhance cyanoacrylate-developed latent prints. A fluorescent light source that will output light around 500 nm is required for this process.

Accepted Deviations:
A rinse of methanol may be used to remove excess dye stain from the item. A heat gun may be used to dry the item before viewing with a Forensic Light Source. ​

Supporting Reference Materials:
  1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
  2. "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
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  • Home
  • About Us
  • Membership
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  • Seminars
  • Webinars
  • Students
  • Resources
  • Vendors
  • Job Opportunities/Training
  • Interactive Chemical Reagent Program
  • ACE-V Methodology