CHESAPEAKE BAY DIVISION - IAI
  • Home
  • About Us
  • Membership
  • Conferences
  • Seminars
  • Webinars
  • Students
  • Resources
  • Vendors
  • Job Opportunities/Training
  • Interactive Chemical Reagent Program
  • ACE-V Methodology

Nile Red

Back to Start

Development Color:
Picture
Vertical Divider
Method to Record:
Picture
Picture
Vertical Divider
Hazard:
Picture
Picture
Vertical Divider
Protective Clothing: 
Picture
Vertical Divider
 Fume Hood Use:
Picture

Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagent
Formula
  1. 100 mg Nile red dye
  2. 1000 ml Ethanol












​
Procedure of Application
  1. Spray, dip, or use a squirt bottle to apply the Nile Red solution to the item.
  2. Examination under a laser or Forensic Light Source at 450 nm to 560 nm. Absorption Max is at 530 nm. Use orange or red colored goggles.
  3. Allow the item to air dry.
  4. Photograph results using an orange or bandpass 550(BP35) barrier filter.




​
​
Development Complete When
The cyanoacrylate has absorbed the dye stain.







​

​



​
Source of Error
Surfaces that absorb too much dye stain will fluoresce too brilliantly to be effective for photography.






​





​
Incompatibilities
Items that inherently fluoresce in the 530 nm to 590 nm range will interfere with the dye stain fluorescence.









​


​
Precautions
Avoid excess build-up of cyanoacrylate, since this may result in ridge detail depicting little contrast to the strongly fluorescent surface.








​


​
Storage Container
Dark stoppered glass or plastic bottles.







​





​
Safety
Fume hood use is required when preparing and applying the reagent. 









​


​
Recommendations
The recommended procedure of application is to immerse the item in a tray of the dye.

A rinse using Methanol may be necessary to reduce excessive staining by the dye.






​


​
Similar Reagent
  • Ardrox
  • Basic Yellow 40
  • Basic Red 28
  • Liqui-Drox
  • M.B.D.
  • Rhodamine 6G
  • Safranin O
  • Thenoyl Europium Chelate

​
​


​
Sequential Reagent
​Not Necessarily in this Order:​
  • M.R.M. 10
  • R.A.M.
  • R.A.Y.









​
Vertical Divider
Chemical Name
Nile Red
​Surface Used On
Non-Porous Surfaces
​
Sensitive To
Cyanoacrylate Deposit
​
Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light
  3. Cyanoacrylate Fuming
  4. Nile Red
  5. Forensic LIght​
  6. R.A.M.
  7. Forensic Light
Vertical Divider
Ridge Detail Visualized by:
Forensic Light Source induced
​
Reagent Applicabilities:
Non-Porous Surfaces
Fluorescent Technique
Post Cyanoacrylate

Other Chemical Name(s):
Nile Blue Oxazone

Working Solution Shelf-life:
Six (6) months

Process Summary:
A fluorescent dye-stain used to enhance cyanoacrylate-developed latent prints. A fluorescent light source that will output light between 450 nm to 560 nm is required for this process. ​

Accepted Deviations:
A number of organic solvents such as n-Heptane, xylene, chloroform, acetone and ethanol may be used to dissolve the Nile red dye. The excitation wavelength may be varied to determine which produces the best fluorescence. ​

Supporting Reference Materials:
  1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
  2. Day, K. and Bowker, W., "Enhancement of Cyanoacrylate Developed Latent Prints Using Nile Red", Jor. Forensic Identification, Vol. 46, No. 2, March/ April 1996, pp. 183-187. 

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
Technical Problems
Problem with website?

Please contact:
Da-il Kim, Webmaster
cbdiaiwebm@gmail.com
Social
Copyright © Company. All Rights Reserved.
  • Home
  • About Us
  • Membership
  • Conferences
  • Seminars
  • Webinars
  • Students
  • Resources
  • Vendors
  • Job Opportunities/Training
  • Interactive Chemical Reagent Program
  • ACE-V Methodology