CHESAPEAKE BAY DIVISION - IAI
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Rhodamine 6G

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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing: 
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 Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagent
Formula
Rhodamine 6G Stock Solution:
1 g Rhodamine 6G dissolved in 1 liter Methanol

Rhodamine 6G Working Solution:
(Combine in the order listed)
3 ml Rhodamine Stock Solution
15 ml Acetone
10 ml Acetonitrile
15 ml Methanol
32 ml Isopropanol
925 ml Petroleum ether



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Procedure of Application
  1. Spray, dip, or use a squirt bottle to apply the Rhodamine solution to the item.
  2. Examination under a laser or Forensic Light Source at 495 nm to 540 nm. Absorption Max is at 525 nm. Use orange or red colored goggles.
  3. Allow the item to air dry.
  4. Photograph results using an orange or bandpass 550(BP35) barrier filter.




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Development Complete When
The cyanoacrylate has absorbed the dye stain.







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Source of Error
Surfaces that absorb too much dye stain will fluoresce too brilliantly to be effective for photography.






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Incompatibilities
Items that inherently fluoresce in the 555 nm range will interfere with the dye stain fluorescence.









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Precautions
Avoid excess build-up of cyanoacrylate, since this may result in ridge detail depicting little contrast to the strongly fluorescent surface.


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Storage Container
Dark stoppered glass or plastic bottles.







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Safety
Fume hood use is required when preparing and applying the reagent. 






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Recommendations
The recommended procedure of application is to immerse the item in a tray of the dye.

A rinse using alcohol may be necessary to avoid excessive staining by the dye.





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Similar Reagent
  • Ardrox
  • Basic Yellow 40
  • Basic Red 28
  • Liqui-Drox
  • M.B.D.
  • Nile Red
  • R.A.Y.
  • Rhodamine 6G
  • Safranin O
  • Thenoyl Europium​ Chelate


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Sequential Reagent
​Not Necessarily in this Order:​
  • M.R.M. 10
  • R.A.M.
  • R.A.Y.






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Chemical Name
Rhodamine 6G
​Surface Used On
Non-Porous Surfaces
​
Sensitive To
Cyanoacrylate Deposit
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Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light
  3. Cyanoacrylate Fuming
  4. Rhodamine 6G
  5. Forensic Light​
  6. R.A.M.
  7. Forensic LIght
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Ridge Detail Visualized by:
Forensic Light Source induced
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Reagent Applicabilities:
Non-Porous Surfaces
Fluorescent Technique
Post Cyanoacrylate

Other Chemical Name(s):
  1. 9-(2-ethoxycarbonyl)phenyl)-3,6-bis(ethyl amino)-2,7-dimethyllxanthylium chloride
  2. Basic red 1

Working Solution Shelf-life:
Six (6) months

Process Summary:
A fluorescent dye-stain used to enhance cyanoacrylate-developed latent prints. A fluorescent light source that will output light between 495 nm and 530 nm is required for this process. 

Accepted Deviations:
The strength (i.e. concentration) of the dye stain may be adjusted to personal preferences. The excitation wavelength may be varied to determine which produces the best fluorescence. ​

Supporting Reference Materials:
  1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
  2. "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
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