CHESAPEAKE BAY DIVISION - IAI
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Basic Red 28

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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing: 
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 Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagent
Formula
Stock Solution (Combine in order listed):
​ 0.2 g Basic Red 28 dye dissolved in 60 ml Propanol, then add 40 ml Acetonitrile.

Working Solution:
5 ml Stock Solution
95 ml Petroleum ether







​
Procedure of Application
  1. Treat item with cyanoacrylate.
  2. Submerge the item in the reagent or use a squirt bottle to apply.
  3. Allow item to air dry.
  4. Distilled water rinse. Again allow item to air dry.
  5. Examination under a laser or Forensic Light Source at 470 nm to 550 nm. Absorption Max is 510 nm. Use orange colored goggles.
  6. Photograph results using an orange or bandpass 550(BP35) barrier filter.


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​
Development Complete When
The cyanoacrylate has absorbed the dye stain.







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Source of Error
DO NOT SPRAY the Basic Red 28 working solution to stain the cyanoacrylate.






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Incompatibilities
Items that inherently fluoresce in the 585 nm range will interfere with the dye stain fluorescence.









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Precautions
Avoid excess build-up of cyanoacrylate, since this may result in ridge detail depicting little contrast to the strongly fluorescent surface.








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Storage Container
Dark stoppered glass bottles with tight-fitting lids.







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Safety
Fume hood use is required when preparing and applying the reagent.









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Recommendations
Under-fume, rather than over-fume, items using cyanoacrylate ester to prevent excess dye adhering to the developed latent prints.

Use a vacuum chamber, if available for the cyanoacrylate fuming.








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Similar Reagent
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  • Basic Yellow 40
  • Liqui-Drox
  • M.B.D.
  • Nile Red
  • Rhodamine 6G
  • Safranin O
  • Thenoyl Europium Chelate











​
Sequential Reagent
​Not Necessarily in this Order:
  • M.R.M. 10
  • R.A.M.
  • R.A.Y.









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Chemical Name
Basic Red 28
​Surface Used On
Non-Porous Surfaces
​
Sensitive To
Cyanoacrylate Deposit
​
Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light
  3. Cyanoacrylate Fuming
  4. Basic Red 28
  5. Forensic LIght
  6. R.A.Y.
  7. Forensic Light
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Ridge Detail Visualized by:
Forensic Light Source induced
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Reagent Applicabilities:
Non-Porous Surfaces
Fluorescent Technique
Post Cyanoacrylate

Other Chemical Name(s):
None

Working Solution Shelf-life:
Six (6) months

Process Summary:
A fluorescent dye-stain used to enhance cyanoacrylate-developed latent prints. A forensic light source that will output light between 470 nm to 550 nm is required for this process. ​

Accepted Deviations:
This reagent can be modified by the addition of Basic yellow 40 dye to provide a wider range of fluorescence. ​

Supporting Reference Materials:
  1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
  2. "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.
  3. Advances in Fingeprint Technology 2nd. Ed., Lee, H.C. & Gaensslen, R.E., CRC Press, Boca Raton, FL., 2001.
  4. Manual of Fingerprint Development Techniques 2nd. Ed., Home Office - Police Scientific Development Branch, White Crescent Press, Ltd., Luton, England, 2001.
  5. Technical Notes, Lightning Powder Co. Inc., Salem, OR., 2001.

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
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