CHESAPEAKE BAY DIVISION - IAI
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Basic Yellow 40

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Development Color:
Picture
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Method to Record:
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Picture
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Hazard:
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Protective Clothing: 
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 Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagent
Formula
Working Solution:
​Take 1 gram Basic Yellow 40 dye dissolved in 500 ml Methanol.






​




​
Procedure of Application
1. a) Tray immersion or aerosol sprayer for 5 seconds.
   b) Water Rinse - Squirt bottle application or gently
        running stream for 10 seconds

2. View under an Ultra-Violet lamp around 365 nm. View using ultra-violet protection goggles.

2a. View under a Forensic Light Source in the 450 nm to 485 nm range. View using yellow colored goggles.

3. Photograph results using a yellow colored or 515(BP 35) bandpass filter.



Development Complete When
The cyanoacrylate has absorbed the dye stain.













​
Source of Error
Too much absorbed dye stain fluoresces too brillantly to be effective for photography.












​
Incompatibilities
Items that inherently fluoresce in the 490 nm range will interfere with the dye stain fluorescence.













Precautions
Avoid excess build-up of cyanoacrylate, since this may result in ridge detail depicting little contrast to the strongly fluorescent surface.












Storage Container
Dark stoppered glass bottles.













​
Safety
Use Ultra-violet protection goggles when working with low-wavelength (i.e. 365 nm) light sources.

Fume hood use is required.











Recommendations
Misting the item with Basic Yellow 40 from an aerosol sprayer cuts down on excessive background staining.





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​
Sequential Reagent
​Not Necessarily in this Order:
  • M.R.M. 10
  • R.A.M.
  • R.A.Y.










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Chemical Name
Basic Yellow 40
​Surface Used On
Non-Porous Surfaces
​
Sensitive To
Cyanoacrylate deposit
​
Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light
  3. Cyanoacrylate Fuming
  4. Basic Yellow 40
  5. Forensic Light
  6. R.A.Y.
  7. Forensic Light
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Ridge Detail Visualized by:
Ultra-Violet light induced

Forensic Light Source induced

Reagent Applicabilities:
Post Cyanoacrylate
Non-Porous surfaces
Fluorescent technique

Other Chemical Name(s):
BY40
Panacryl Brilliant Flavine 10GFF
Maxilon Flavine 10GFF
Yellow Brilliance

​Working Solution Shelf-life:
Six (6) months

Process Summary:
A fluorescent dye-stain used to enhance cyanoacrylate-developed latent prints. A forensic light source or ultra-violet lamp that will output light between 365 nm and 500 nm is required for this process. ​

Accepted Deviations:
The working solution may be applied by either dipping, spraying or squeegee bottle. Some researchers advise to allow the cyanoacrylate-developed prints to "sit" overnight prior to applying the dye stain.

Supporting Reference Materials:
  1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
  2. "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.
  3. Advances in Fingeprint Technology 2nd. Ed., Lee, H.C. & Gaensslen, R.E., CRC Press, Boca Raton, FL., 2001.
  4. Manual of Fingerprint Development Techniques 2nd. Ed., Home Office - Police Scientific Development Branch, White Crescent Press, Ltd., Luton, England, 2001.
  5. Technical Notes, Lightning Powder Co. Inc., Salem, OR., 2001.

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
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