CHESAPEAKE BAY DIVISION - IAI
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R.A.M.

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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing: 
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 Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagent
Formula
1. Rhodamine 6G Stock Solution:
1 g Rhodamine 6G dissolved in 1 liter Methanol

2. M.B.D. Stock Solution:
1 g M.B.D. dissolved in 1 liter Acetone

3. R.A.M. Working Solution:
(Combine in the order listed)
3 ml Rhodamine Stock Solution
2 ml Ardrox P133D
7 ml M.B.D. stock Solution
20 ml Methanol
10 ml Isopropanol
8 ml Acetonitrile
950 ml Petroleum ether
Procedure of Application
  1. Spray, dip, or use a squirt bottle to apply R.A.M. to the item.
  2. Examination under a laser or Forensic Light Source at 415 nm to 530 nm. Absorption Max is 460 nm. Use orange colored goggles.
  3. Photograph results using orange barrier filter.





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Development Complete When
The cyanoacrylate has absorbed the dye stain.







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Source of Error
Surfaces that absorb too much dye stain will fluoresce too brilliantly to be effective for photography.






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Incompatibilities
Items that inherently fluoresce in the 555 nm range will interfere with the dye stain fluorescence.









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Precautions
Avoid excess build-up of cyanoacrylate, since this may result in ridge detail depicting little contrast to the strongly fluorescent surface.

'Pooling' may result if the item is immersed into a solution of the dye-stain.





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Storage Container
Dark stoppered glass or plastic bottles.







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Safety
Fume hood use is required when preparing and applying the reagent. 









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Recommendations
The recommended procedure of application is the wash bottle method.

Allow the cyanoacrylate treated item to sit overnight prior to dye staining.

A rinse using Petroleum ether may be necessary to avoid excessive staining by the dye.



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Similar Reagent
  • Ardrox
  • Basic Yellow 40
  • Basic Red 28
  • Liqui-Drox
  • M.B.D.
  • M.R.M. 10
  • Nile Red
  • R.A.Y.
  • Rhodamine 6G
  • Safranin O
  • Thenoyl Europium​ Chelate


​
Sequential Reagent
​Not Necessarily in this Order:​
  • M.R.M. 10
  • R.A.Y.








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Chemical Name
R.A.M.
​Surface Used On
Non-Porous Surfaces
​
Sensitive To
Cyanoacrylate Deposit
​
Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light
  3. Cyanoacrylate Fuming
  4. R.A.M.
  5. Forensic Light​
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Ridge Detail Visualized by:
Forensic Light Source induced
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Reagent Applicabilities:
Non-Porous Surfaces
Fluorescent Technique
Post Cyanoacrylate

Other Chemical Name(s):
None

Working Solution Shelf-life:
One (1) month

Process Summary:
A mixture of fluorescent dye-stains used to enhance cyanoacrylate-developed latent prints. A fluorescent light source that will output light between 415 nm and 530 nm is required for this process. ​

Accepted Deviations:
The excitation wavelength may be varied to determine which produces the best fluorescence. ​

Supporting Reference Materials:
  1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.7.
  2. "Chemical Formulas and Processing Guide for Developing Latent Prints", U.S. Dept. of Justice, pg. 47-48, 1994.
  3. Lennard, C.J. & Margot, P.A., "Sequencing of Reagents for the Improved Visualization of Latent Fingerprints", Jor. Forensic Identification, Vol. 38, No. 5, pp. 197-210, Sept./Oct. 1988

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
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