CHESAPEAKE BAY DIVISION - IAI
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R.A.Y.

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Development Color:
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Method to Record:
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Hazard:
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Protective Clothing: 
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 Fume Hood Use:
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Formula
Procedure of Application
Development Complete When
Source of Error
Incompatibilities
Precautions
Storage Container
Safety
Recommendations
Similar Reagent
Sequential Reagent
Formula
R.A.Y. Working Solution (Combine in the order listed): 
  1. 0.5 g Basic Yellow 40 dye
  2. 10 ml Glacial acetic acid
  3. 0.05 g Rhodamine 6 dye
  4. 4 ml Ardrox P133D
  5. 450 ml Isopropanol - or - denatured Ethanol
  6. 40 ml Acetonitrile






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Procedure of Application
  1. Spray, dip, or use a squirt bottle to apply R.A.Y.
  2. Examination under a laser or Forensic Light Source at 450 nm to 550 nm. Use orange or red colored goggles.
  3. Photograph results using orange or red barrier filter.






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Development Complete When
The cyanoacrylate has absorbed the dye stain.







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Source of Error
Surfaces that absorb too much dye stain will fluoresce too brilliantly to be effective for photography.






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Incompatibilities
Items that inherently fluoresce in the 490 nm to 555 nm range will interfere with the dye stain fluorescence.









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​
Precautions
Avoid excess build-up of cyanoacrylate, since this may result in ridge detail depicting little contrast to the strongly fluorescent surface.


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Storage Container
Dark stoppered glass or plastic bottles.







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Safety
Use Ultra-violet protection goggles when working with long-wavelength ultra violet light sources.

Fume hood use is required when preparing and applying the reagent.






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Recommendations
The recommended procedure of application is to immerse the item in a tray of the dye.

A rinse using alcohol may be necessary to avoid excessive staining by the dye.





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Similar Reagent
  • Ardrox
  • Basic Yellow 40
  • Basic Red 28
  • Liqui-Drox
  • M.B.D.
  • M.R.M. 10
  • Nile Red
  • R.A.Y.
  • Rhodamine 6G
  • Safranin O
  • Thenoyl Europium​ Chelate


​
Sequential Reagent
​Not Necessarily in this Order:​
  • M.R.M. 10
  • R.A.M.








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Chemical Name
R.A.Y.
​Surface Used On
Non-Porous Surfaces
​
Sensitive To
Cyanoacrylate Deposit
​
Abridged Reagent Sequence
  1. Visual Examination
  2. Forensic Light
  3. Cyanoacrylate Fuming
  4. Rhodamine 6G
  5. ​Forensic Light
  6. R.A.Y.
  7. Forensic Light​
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Ridge Detail Visualized by:
Forensic Light Source induced
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Reagent Applicabilities:
Non-Porous Surfaces
Fluorescent Technique
Post Cyanoacrylate

Other Chemical Name(s):
None

Working Solution Shelf-life:
Six (6) months

Process Summary:
A mixture of fluorescent dye-stains used to enhance cyanoacrylate-developed latent prints. A fluorescent light source that will output light between 365 nm and 550 nm is required for this process. ​

Accepted Deviations:
The excitation wavelength may be varied to determine which produces the best fluorescence. ​

Supporting Reference Materials:
  1. Minutiae Magazine, Summer Special 1994, Issue No. 24, pg.5.

Address
Chesapeake Bay Division - International Association for Identification   

P.O. Box 71804, 
Henrico, VA 23255
Email
cbdiaisec@gmail.com
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  • Home
  • About Us
  • Membership
  • Conferences
  • Seminars
  • Webinars
  • Students
  • Resources
  • Vendors
  • Job Opportunities/Training
  • Interactive Chemical Reagent Program
  • ACE-V Methodology